International Journal of Engineering
Trends and Technology

Research Article | Open Access | Download PDF

Volume 67 | Issue 8 | Year 2019 | Article Id. IJETT-V67I8P207 | DOI : https://doi.org/10.14445/22315381/IJETT-V67I8P207

Effect of Microbial Lipase Enzymes on Wettability, Interfacial Tension and Adhesion of Crude oil/brine/solid Interactions


Ndubuisi Gabriel Elemuo, Sunday Sunday Ikiensikimama, Chikwendu Ebenezer Ubani, Evans Chidi Egwim, Christopher Adigwe

Citation :

Ndubuisi Gabriel Elemuo, Sunday Sunday Ikiensikimama, Chikwendu Ebenezer Ubani, Evans Chidi Egwim, Christopher Adigwe, "Effect of Microbial Lipase Enzymes on Wettability, Interfacial Tension and Adhesion of Crude oil/brine/solid Interactions," International Journal of Engineering Trends and Technology (IJETT), vol. 67, no. 8, pp. 49-52, 2019. Crossref, https://doi.org/10.14445/22315381/IJETT-V67I8P207

Abstract

LPE1 and LPE2 are lipase enzymes produced from crude oil contaminated soil isolates: Verticillium sp. and Penicillum sp. respectively. The enzymes were produced in broth medium using standard microbiological technique. The influence of the produced enzymes on wettability, interfacial tension and adhesion behavior of crude oil/brine/solid system were investigated. These properties were evaluated using standard methods. The experimental result reveal that the produced lipases changed the wettability nature of oil/brine/solid system to a water wet by the contact angle measurement. The microbial lipases also changed the adhesion behavior of the oil on glass slide from adhesive behavior to non-adhesion. In the case of interfacial tension, no significant change was observed when the lipase enzymes were introduced into the system. These findings suggest the possible application of the microbial lipases for enhanced oil recovery.

Keywords

LPE; lipase enzymes; wettability; interfacial tension, adhesion, oil/brine/solid system.

References

[1] J. O. Osuoha, B. W. Abbey, E. C. Egwim, E.O. Nwaichi. “Production and Characterization of Tyrosinase Enzyme for Enhanced Treatment of Organic Pollutants in Petroleum Refinery Effluent”. Society of Petroleum Engineers. doi:10.2118/198791-MS, August, 2019.
[2] W. G. Anderson. “Wettability Literature Survey - Part 2: Wettability Measurement”, JPT: pp 1246-62, 1986.
[3] E. Omar. “Surfactant Enhanced Oil Recovery by Wettability Alteration in Sandstone Reservoir”, A Thesis Presented to the Faculty of the Graduate School; Missouri University of Science and Technology, 2012
[4] K. Spildo. “Wettability and two-phase flow in capillary systems” University of Bergen, Bergen, Norway, 1999.
[5] H. Nasiri. “Enzymes for enhanced oil recovery (EOR)”, The University of Bergen, 2011.
[6] J. S. Buckley, K. Takamura and N. R. Morrow. “Influence of electrical surface charges on the wetting properties of crude oils”. SPE Reservoir Engineering, 1989
[7] J. S. Buckley. “Mechanisms and consequences of wettability alteration by crude oils”, Heriot-Watt University, Edinburgh, 1996
[8] J. S. Buckley and T. Fan. “Crude oil/brine interfacial tension”, International Symposium of the Society of Core Analysts, Toronto, Canada, 2005
[9] J. O. Osuoha, C. U. Iheka, P. U. Amadi, I. E. Archibong, O. T. Adeoti. “Distribution and Toxicological Risk Evaluation of Pb, Cd, As and Zn from Surface Soils of Selected Marts in Port Harcourt, Rivers State, Nigeria”, Journal of Chemical Health Risks, JCHR, 8 (4), 255-264, 2018.
[10] Y. Liu and J. S. Buckley. “Evolution of wetting alteration by adsorption from crude oil in SPEFE. SPE”, Texas, USA, 1997
[11] F. K. Hansen and G. Rodsrud. “Surface tension by pendant drop 1. A fast standard instrument using computer image analysis”. Journal of Coll. Int. Sci., 141, 1991
[12] J. N. Oyibo, M.O. Wegwu, A.A. Uwakwe, and J. O. Osuoha. “Analysis of total petroleum hydrocarbons, polycyclic aromatic hydrocarbons and risk assessment of heavy metals in some selected finfishes at Forcados Terminal, Delta State, Nigeria”, Environmental Nanotechnology, Monitoring and Management, vol 9, pp. 128-135, 2018.

Time: 0.0013 sec Memory: 36 KB
Current: 1.89 MB
Peak: 4 MB